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  • 6 - 9 Years
  • Posted : above 1 month

Job Description:

JOB PURPOSE (Briefly describe the objectives of the job)

Syngene is seeking a candidate who will be a part of the mammalian expression team The candidate needs to be well trained in routine mammalian cell culture practices like establishment of cell banks, expansion and scale up of cell lines, serum free adaptation and characterization of cell lines The Candidate should also have basic molecular biology experience

Candidate must possess a broad knowledge of fermentation process and be well trained in operating wave bioreactors and /or stirred tank reactors and should have experience with mammalian expression systems (CHO, HEK, etc)

Experience with protein expression in human cell lines other than HEK and Insect cells would be considered a plus


Under the guidance of reporting manager design, plan and execute experiments along with compilation and analysis of data with minimal error
Perform transient expressions in mammalian cell lines like HEK, CHO
Plan and perform protein expression at different scales like microtitre plates , shake flask and bioreactor
Perform optimization of media /Feed to improve productivity in batch and fed-batch culture
Perform serum free adaptations and express proteins in batch /fed batch mode using stable clones
Scale up plasmid DNA for transfection depending on the scale of the expression batch
Analysis of expression titers using OCTET, PA-HPLC , western blot
Perform small scale purification using affinity chromatography
Collaborate with colleagues with respect to information/knowledge sharing
Adhere to all EHS requirements including wearing PPEs and safe working practices
Efficient record keeping and data management


WORK EXPERIENCE 6-9 years relevant work experience if the candidate has post graduate education

Profile Summary:

Employment Type : Full Time
Eligibility : Any Graduate
Industry : Healthcare, Biotechnology/Pharmaceutical/Medicine
Functional Area : Bio Tech/R&D/Scientist
Role : Bio-Technology Research
Salary : As per Industry Standards
Deadline : 29th Jan 2020

Key Skills:

Company Profile:

Syngene International Ltdarfix

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